Cryopreservation of immature porcine oocytes by Cryotop
نویسندگان
چکیده
منابع مشابه
Cryotop and development of vitrified immature bovine oocytes
The effectiveness of different cryodevices (open-pulled straw (OPS), electron microscopy grid (EMG), and Cryotop was evaluated for vitrification of immature bovine oocytes. Polar body, metaphase II stage (MII), survivability, and subsequent developmental rates were determined. Only oocytes with four or five layers of cumulus cells were used. Oocytes were equilibrated in two vitrification soluti...
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Cryopreservation of normal, lipid-containing porcine oocytes has had limited practical success. This study used solid surface vitrification (SSV) of immature germinal vesicle (GV) and mature meiosis II (MII) porcine oocytes and evaluated the effects of pretreatment with cytochalasin B, cryoprotectant type (dimethylsulfoxide (DMSO), ethylene glycol (EG), or both), and warming method (two-step ve...
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In vitro maturation of vitrified immature germinal vesicle (GV) oocytes is a promising fertility preservation option. We analyzed the ultrastructure of human GV oocytes after Cryotop vitrification (GVv) and compared it with fresh GV (GVc), fresh mature metaphase II (MIIc) and Cryotop-vitrified mature (MIIv) oocytes. By phase contrast microscopy and light microscopy, the oolemmal and cytoplasmic...
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Porcine ovaries contain a large number of oocytes at different stages of growth, most of which degenerate either before or at various stages of growth. To utilize those potential female gametes stored in the ovary, it is important to develop culture system that can provide a suitable alternative environment for oocytes to achieve full growth and competences to undergo meiotic maturation, fertil...
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Joining immature gamete cryopreservation and germinal vesicle transplantation (GVT) technique could greatly improve assisted reproductive technologies in animal breeding and human medicine. The present work was aimed to assess the most suitable cryopreservation protocol between slow freezing and vitrification for immature denuded bovine oocytes, able to preserve both nuclear and cytoplasmic com...
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ژورنال
عنوان ژورنال: TAP CHI SINH HOC
سال: 2014
ISSN: 0866-7160,0866-7160
DOI: 10.15625/0866-7160/v35n4.3784